FastQCFastQC Report
Fri 16 Mar 2018
HM7TTBGX5_n02_at21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM7TTBGX5_n02_at21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61342691
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG3517570.5734293593347576No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2515080.4100048365990335No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC2449150.39925701987870077No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2107400.3435454111395276No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1872220.3052066952850177No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA1628330.2654480873687136No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1451810.2366720429659664No Hit
TGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCC1311190.21374836653318652No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1300600.21202199949134937No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA1276840.20814867740314819No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1272990.20752105576848592No Hit
TGGGTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCG1231360.20073459118381357No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC1123110.18308782704038856No Hit
GTGGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATG1119480.1824960694991356No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1102900.17979322100492787No Hit
GTTGTGGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGG1091140.17787612219359597No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1086580.17713275734838563No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG1015850.16560245131730528No Hit
GAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTG981130.1599424453028968No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC943610.15382598719055215No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA924890.1507742788786361No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA911400.1485751578782222No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG906770.14782038173056347No Hit
GAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGG902820.14717645823526065No Hit
GTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCA886180.14446382862466858No Hit
TCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTAT877610.1430667591677711No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG850900.1387125321906729No Hit
TATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCAC847860.13821695562719932No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG845750.13787298636768314No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC810600.1321428823525202No Hit
CGGGGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCG810330.13209886732879064No Hit
CTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCACTGTCATGACGC778710.12694421899424008No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA771010.1256889757249156No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC759340.12378654858816024No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA756700.12335617946724899No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT755920.12322902495425249No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC752850.12272855783258677No Hit
GCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAA744860.12142603916740465No Hit
CTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTC743420.12119129237418032No Hit
CCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATT728300.11872645104532503No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC726690.11846399108901173No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA714810.11652733004491114No Hit
CTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATC702210.11447329560419839No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT697130.11364516108365705No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA692480.1128871245638702No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT687870.11213560878833959No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC685990.11182913380829675No Hit
CGGACGGGGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGT673180.10974086546023877No Hit
GAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAG668530.10898282894045194No Hit
TGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCACTGT667840.10887034610203195No Hit
GGAAAACTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTC652620.10638920291253606No Hit
CCTCATTATGCGACCAGGTACCGATGACACTCCTATCTCGACACCTTGTC643150.10484541670987338No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG635810.10364886013885502No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA630310.10275225780362325No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG617260.10062486499002792No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA616790.1005482462450172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG411450.071.899243
GTTGTGG253850.068.019761
GGGTCTG440900.066.845342
TGGGTCT305400.064.554641
TGTGGCG297250.058.942853
CTTGTTA221900.057.5002141
GCCTGTT44550.055.7614971
CGATGGG103400.052.8547821
GGGCACT28350.051.0512121
TTACGAC249300.051.0297135
GTTACGA249350.050.9227184
CCTGGCC833150.048.747985
CACCTGG854650.048.1799933
ACCTGGC884250.046.402334
TCACCTG889000.046.0628622
TGGCCCG890350.045.6042147
GCACGAC147500.045.327648
CTGGCCC908700.044.714356
TCTGGAG666700.044.6462065
ATCAAAG276050.043.5047076
Produced by FastQC (version 0.11.2)