FastQCFastQC Report
Thu 15 Mar 2018
HM7TTBGX5_n02_ac22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM7TTBGX5_n02_ac22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68033135
Sequences flagged as poor quality0
Sequence length150
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1964820.28880338970120956No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1850240.2719615963603617No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG1779310.2615357942861225No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1682600.24732066220379234No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC1505450.22128187977814048No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1366280.20082567119683667No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1311010.19270168866979304No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1038650.15266825496135084No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG929870.1366789873787236No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA913050.13420666267988385No Hit
TGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCC894150.13142860460568223No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT857400.1260268250169568No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA857130.12598713847303966No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG767930.11287588026040547No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC753700.11078425240877111No Hit
TATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCAC709790.10433004447024234No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA702880.10331436292036227No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA702590.10327173663245122No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG700510.10296600325708936No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG259950.060.5326463
TGGGTCT202950.049.2450941
GAGTCTA255950.044.2823832
CTTGTTA235000.043.6013151
CGCGGAT81950.043.4929431
TCCACTC610500.042.6858862
AGTCTAA239450.041.4139943
GTTGTGG190200.040.4696771
GCTTGTA696950.039.9437757
CCTGGCC544100.039.8134965
ACTCCTG668150.039.440775
TCTGGAG401750.039.3957865
TTACGAC260500.039.324895
GGAGTCT295800.038.6071361
ATCGAAG1096050.038.466486
GTTACGA266500.038.323874
GTCCAAT124550.037.982561
CTTGTAA739000.037.8377238
TGGCCCG574950.037.8309027
TCTAACG273450.036.3033685
Produced by FastQC (version 0.11.2)