FastQCFastQC Report
Thu 15 Mar 2018
HM7TTBGX5_n02_ac21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM7TTBGX5_n02_ac21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82529538
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG2428700.29428251494634566No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2264190.27434904579255004No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2088500.2530609101434689No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC2053640.2488369679229272No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG1885660.2284830432468918No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1468050.1778817664046538No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC1458620.17673914520156409No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1445980.17520757234821793No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1360060.16479675434509278No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1316910.15956832328323467No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1309110.1586232071237331No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1244720.15082115205830912No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC1153190.13973057743277323No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC1104840.13387206893124737No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC1086560.13165710439333855No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA1050580.1272974531857915No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA971890.11776268516128129No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT969420.11746339837743912No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC928410.11249426841575194No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC927310.11236098280351454No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG922210.11174302223768658No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA915170.1108899943193672No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA913890.11073489833421823No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA898860.1089137321961017No Hit
GTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCA888630.10767417600229387No Hit
TGGGTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCG850430.10304553019550407No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG362250.062.481323
TCCACTC848100.052.732152
CTTGTTA396050.052.1588971
TGGGTCT275400.051.4789471
ACTCCTG895050.050.2625735
TTACGAC433150.047.681395
CCTGGCC805900.046.8481645
GTTACGA442950.046.6337134
CGATGGG105000.046.1502041
CTCCTGG1018100.044.4953736
TGGCCCG847150.044.460427
GAGTCTA332400.043.7573852
GTCCAAT186900.042.53121
TCTGGAG535600.042.232685
AGTCTAA281700.039.266173
ACGACTT525800.039.1186457
CCGGCAT111550.039.0511361
TCCTGGT1199600.038.0099147
ATCAAAG378750.038.004556
GTTGTGG307600.037.8036771
Produced by FastQC (version 0.11.2)