FastQCFastQC Report
Thu 15 Mar 2018
HM7TTBGX5_n01_bt22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM7TTBGX5_n01_bt22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75415600
Sequences flagged as poor quality0
Sequence length150
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA1629120.21601896689809533No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT1617530.21448214958178416No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1534470.2034685131458213No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1410600.18704352945544425No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG1355610.17975193461299785No Hit
GTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGTGAGGCGGGG1301690.17260222023029717No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT1265390.1677888924837832No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1264270.16764038209601198No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG1230180.16312009716822512No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1212460.16077045067598747No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT1146420.15201364173990525No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1116680.14807016055033706No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1092610.14487851319886072No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA1005970.13339017391627198No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT962830.12766987201586938No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA932410.12363622380515438No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT899740.1193042288332918No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA869320.11527058062257675No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC855450.1134314385883027No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC834410.11064156487517172No Hit
GTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG825930.1095171290820467No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA790630.10483639989604272No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT784020.10395992341107145No Hit
GTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC756990.10037578432048541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA138700.056.0285221
GGAGTCT270700.053.264761
GAGTCTA277050.052.077072
AGTCTAA277950.050.3278243
GGTCTGG174350.049.3868453
GTCCAAT49250.046.795911
TTACGAC166900.046.5442435
TGTTACG167150.046.5178263
GCTTGTA415250.044.2797247
CTTGTAA440750.041.7506648
AGCGCTT449300.040.860564
TACCACT534000.040.4323437
GTTACGA193350.040.2887424
CCTGGCC463950.039.7875985
ACCACTA553500.038.955978
CGGATGT43950.038.65943
ATACCAC569600.037.956366
TCTGGAG226900.037.88535
TTGTAAT487200.037.7257929
GTCAACT671600.037.175957
Produced by FastQC (version 0.11.2)