HM7TTBGX5_n01_bt21.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HM7TTBGX5_n01_bt21.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61739269
Sequences flagged as poor quality	0
Sequence length	150
%GC	56

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA	140314	0.22726864485551326	No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC	127596	0.20666911362361612	No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC	119026	0.1927881588620688	No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT	109123	0.17674812443924465	No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT	107153	0.17355728653023086	No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT	101455	0.16432815231421027	No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT	95108	0.1540478232743572	No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG	94627	0.15326874051586195	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT	93185	0.15093311195505085	TruSeq Adapter, Index 1 (97% over 36bp)
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA	89531	0.14501467453396638	No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT	89241	0.14454495727832475	No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA	85839	0.13903468795524612	No Hit
GTGAAATACCACTACTCTCATCGTTTTTTCACTGACCCGGTGAGGCGGGG	84661	0.13712666406853632	No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA	82014	0.13283927932480055	No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA	79705	0.12909935814108844	No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC	76467	0.12385472202464853	No Hit
GTCGTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGG	75144	0.12171183951011795	No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT	73073	0.1183574104189669	No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT	65864	0.10668088732958597	No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA	64075	0.10378321777667955	No Hit
GTTGTCTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC	63941	0.10356617601027962	No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT	62862	0.10181850387635785	No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG	62703	0.10156096924309227	No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA	62535	0.10128885717775504	No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC	61828	0.10014371890279426	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTCCAAT	4875	54.809986	1
GGAGTCT	23605	52.69249	1
CTTGTTA	10650	52.27455	1
GAGTCTA	24015	51.65535	2
AGTCTAA	24120	49.847744	3
TTACGAC	12320	45.698467	5
GGTCTGG	10535	43.873955	3
CCTGGCC	39340	43.75733	5
GCTTGTA	22470	42.197075	7
TGTTACG	13610	41.15549	3
GTGCGTC	5390	40.60566	9
GTTACGA	14405	39.08401	4
CTTGTAA	24715	38.50992	8
TCTAACG	32095	37.506325	5
GTCAACT	58680	37.19964	7
TGGCCCG	47520	36.22453	7
TTGTAAT	26600	35.726795	9
AGCGCTT	26540	35.672245	4
CGCGGAT	4930	35.645386	1
TACCACT	40345	35.457474	7

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content