FastQCFastQC Report
Thu 15 Mar 2018
HM7TTBGX5_n01_bc22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM7TTBGX5_n01_bc22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64439244
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2559040.3971244603676604No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG2311130.3586525627147333No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2304850.35767800131236793No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC2291630.355626456449427No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1905770.29574679678116644No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1798050.2790302754017412No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1625200.252206559096193No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1611090.2500168996396047No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC1360400.21111358786270054No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC1307130.20284688628563052No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1184350.18379327975976875No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA1183920.18372655023699533No Hit
TGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCC1077020.16713728050564963No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC1029010.15968685169552888No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA1019300.15818000596034304No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC1008480.15650090494543975No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT987100.15318305099917062No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG932370.14468977941454433No Hit
TGGGTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCG931150.14450045379179186No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC898320.1394057323205095No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC895110.1389075886737591No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA887740.13776387569041001No Hit
GTTGTGGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGG882980.13702519539180194No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG874060.13564094575659516No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC856870.13297331669502516No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA850040.13191340357748454No Hit
GTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCA849800.13187615919268078No Hit
GTGGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATG846610.131381119244664No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA817120.12680471546190084No Hit
GAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGG807600.12532735486468463No Hit
GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT792400.12296854382711257No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA784300.12171154583998534No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT766840.1190020168455111No Hit
TCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTAT761190.11812522195325569No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT751280.11658733923073338No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACT745490.11568881844734244No Hit
TATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCAC742130.11516739706008965No Hit
GGAAAACTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTC732060.11360468474769815No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG730280.11332845556040352No Hit
GCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAA725860.11264253814026744No Hit
GAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAG702690.10904690315733687No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT699580.10856427800425468No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA699040.10848047813844619No Hit
TGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTT693610.1076378239322609No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT688750.10688362513998458No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT685630.10639944813753557No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA663340.10294037589888548No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC653820.10146301530166928No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG646310.10029757642718465No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG646130.10026964313858183No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG337900.067.413553
TGGGTCT262250.055.4463271
GTTGTGG246950.053.9528851
GGGTCTG440450.052.0615582
CTTGTTA269950.051.543721
GTTACGA293350.047.562674
TTACGAC297550.046.9155125
GTCCAAT120950.046.6833651
TGTGGCG299500.044.975493
CGGGTCT121900.042.656521
CCTGGCC821750.042.6052555
CGATGGG107300.042.352751
GGAGTCT204650.042.0188871
GAGTCTA209900.040.816792
AGTCTAA147450.040.525863
TCTGGAG566550.040.295515
TGGCCCG875300.039.9735037
CCGGCAT84550.039.4384041
CGAAGGA892250.038.972228
ATCGAAG906000.038.7307246
Produced by FastQC (version 0.11.2)