FastQCFastQC Report
Thu 15 Mar 2018
HM7TTBGX5_n01_at22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM7TTBGX5_n01_at22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63031388
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG3007190.4770940471753533No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2732720.4335490755812009No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2462370.3906577465817507No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1976090.31350888227306684No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC1652550.2621789004551193No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1579830.2506417913563953No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1509630.2395044830680232No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1463240.2321446578330149No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA1328190.2107188247226921No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1308590.20760926286440018No Hit
TGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCC1248590.19809019595126162No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC1225030.19435237567670252No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA1190940.18894395915888762No Hit
TGGGTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCG1083600.17191434845128273No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC1068740.16955679287912873No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1024490.16253648103068902No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG986370.15648870051854166No Hit
GTTGTGGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGG969020.15373610366949242No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC959210.15217973622919426No Hit
GTGGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATG952910.1511802342033147No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA940410.1491970952630775No Hit
GTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCA900670.14289229994427538No Hit
GAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTG899280.142671774894121No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG882400.13999374406922468No Hit
GAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGG868170.13773613869965867No Hit
TATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCAC857220.13599890898801087No Hit
TCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTAT854200.13551978262004954No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA851230.1350485888078492No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA826820.13117591508535398No Hit
CTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTC824400.13079197938652407No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC818890.1299178117416675No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC815280.12934508121572702No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC799390.12682411499489746No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT794250.12600864826267194No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA793430.12587855434819237No Hit
CCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATT788900.1251598647962504No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA782640.12416670881497961No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT760070.12058595314448732No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG756100.11995610821706798No Hit
CTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCACTGTCATGACGC748350.11872656207412091No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC744270.11807926552402749No Hit
GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT729120.11567570112846No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT727850.11547421421213189No Hit
GCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAA721910.11453182658773117No Hit
TGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCACTGT721560.11447629869740454No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC707150.11219013612709908No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA702710.11148572517552682No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA702710.11148572517552682No Hit
GGAAAACTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTC679550.10781136534705534No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT679340.10777804861285935No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA667690.10592976312055828No Hit
GTAACTTTCACTTGAAATAATAGTGACATGTATCGATTATAATTTTGCTT661430.10493660713928749No Hit
CTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATC661000.10486838715974332No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG655910.10406085298327875No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG653160.10362456241642655No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT637520.10114325897440178No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA633920.10057211495961345No Hit
CGGACGGGGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGT633460.10049913544661272No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC630860.10008664254704339No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC630860.10008664254704339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTGG197700.072.602361
GGTCTGG360950.069.1928563
GGGTCTG401500.062.1873972
TGGGTCT277150.060.5467951
TGTGGCG264800.054.9208683
CTTGTTA228000.054.5516171
CGATGGG96500.053.660131
GCCTGTT46500.051.8850171
ATCAAAG240250.048.815366
GTTACGA259050.048.385674
TTACGAC261200.048.097655
CCTGGCC894300.047.827535
TCCAATC99400.047.514522
GTCCAAT101700.046.738321
CTGGCCC948450.045.096626
GATGGGT128450.045.064112
CACCTGG974550.044.5173233
TGGCCCG968000.044.1702967
AGTCTAA116550.043.3639533
ACCTGGC1000500.043.312064
Produced by FastQC (version 0.11.2)