FastQCFastQC Report
Thu 15 Mar 2018
HM7TTBGX5_n01_at21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM7TTBGX5_n01_at21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61342691
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG3573720.5825828540844418No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2705990.44112671874795967No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2199920.3586278926041898No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC1926130.313995028356353No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1760040.28691926801841805No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA1661830.270909210683307No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1457870.23765993572078536No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1456780.2374822454398031No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1376040.22432012315860092No Hit
TGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCC1368480.22308770249417328No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA1321090.21536225073660364No Hit
TGGGTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCG1211040.19742205310164826No Hit
GTTGTGGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGG1174210.1914180778277236No Hit
GTGGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATG1166860.19021989107064116No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC1131310.18442457961291592No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1127140.18374479202420382No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1077510.17565417858828528No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA1034560.1686525294431573No Hit
GAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTG1025910.16724241849774735No Hit
GTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCA990650.16149438243587977No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC979620.15969628720722406No Hit
GAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGG945700.15416669607794023No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG945490.15413246217059504No Hit
TCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTAT933450.15216971814946953No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA913060.14884576876485578No Hit
TATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCAC908460.14809588317538921No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG875920.14279125772294535No Hit
CTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCACTGTCATGACGC864270.1408920909583181No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG855950.13953577615302204No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT836200.136316158676508No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC835950.13627540402490657No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA812090.1323857800760648No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC782460.12755553876826173No Hit
CTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTC770130.12554551935127853No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC769130.12538250074487278No Hit
CCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATT761650.12416312156895758No Hit
TGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCACTGT744580.12138039395761101No Hit
GCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAA729460.11891555262875572No Hit
CTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATC724090.11804014271235672No Hit
CCTCATTATGCGACCAGGTACCGATGACACTCCTATCTCGACACCTTGTC723180.11789179578052746No Hit
CGGACGGGGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGT711110.11592416120120978No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA711080.11591927064301759No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA709610.11567963329160111No Hit
GAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAG704090.11477977058424124No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG691200.11267846074767082No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT685160.11169382836497994No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA684500.11158623608475214No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC681070.11102708226478034No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT680420.11092112017061657No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA665360.10846605995814562No Hit
GGAAAACTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTC660400.10765748767037299No Hit
CTAAGATCAAAGTGTAGTATCTGTTCTTATCAGCTTAATCTCTGATATGT659190.107460235156622No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA652240.10632725584210187No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT647700.10558715136901967No Hit
AGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGA645310.10519753689970987No Hit
GAGGAGTCCTGGCTGATGCTTGGCGGTGGCGACCGAGCGTCCAGGCCAAG644590.1050801635030977No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC643730.10493996750158875No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA636730.10379883725674832No Hit
GCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGAC632310.10307829501643481No Hit
CGGGGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCG631210.10289897454938844No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC627580.10230721700813548No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG625150.10191108179456947No Hit
CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC623540.10164862183825617No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT621280.10128019978777912No Hit
GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT621150.10125900736894637No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA616520.10050423122128763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTGG238950.075.232271
GGTCTGG390450.070.530133
GGGTCTG422100.065.020362
TGGGTCT292600.063.4317441
TGTGGCG297150.061.3959243
GCCTGTT45600.060.1764681
CTTGTTA211500.058.1626361
CGATGGG101000.053.553161
GTTACGA238050.051.7475054
TTACGAC240450.051.1711165
CCTGGCC864250.049.082985
CACCTGG881800.048.6042753
ATCAAAG265800.047.9700856
ACCTGGC896700.047.7001764
CTGGCCC908250.046.6654556
GCACGAC147750.046.3398488
TGGCCCG918550.046.1491937
TCACCTG929200.045.9160272
GGGCACT28400.045.9014931
GATGGGT133250.045.1160552
Produced by FastQC (version 0.11.2)