FastQCFastQC Report
Thu 15 Mar 2018
HM7TTBGX5_n01_ac22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM7TTBGX5_n01_ac22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68033135
Sequences flagged as poor quality0
Sequence length150
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2219850.3262895352389685TruSeq Adapter, Index 7 (97% over 36bp)
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2041860.30012728356557433No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1883770.27689007716607505No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG1805400.26537069032611826No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1565560.23011728035169923No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1444650.2123450580367934No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1306310.19201084883123495No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC1170740.17208379416882671No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1115350.1639421731778199No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1019070.14979024559135778No Hit
TGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCC925800.13608074947597226No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA869920.1278671047571158No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT864730.12710424119070804No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG792690.11651528332480929No Hit
TATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCAC773480.11369165921870276No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC752750.11064461456906256No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA741730.1090248156284434No Hit
GTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCA735850.10816053089424735No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG728440.10707135574452066No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA723640.10636581718599326No Hit
TGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCACTGT717580.10547507475585244No Hit
CCGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGGCCCCGTCCGGGGGCCC714110.104965029172917No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG707270.10395963672701544No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT693200.101891526827332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG258450.056.8544123
GTTGTGG170450.046.3497961
TGGGTCT202650.045.1331181
GAGTCTA270250.044.6487122
CTTGTTA239550.044.4942321
CGCGGAT74850.042.334951
TCCACTC623700.042.0402072
AGTCTAA248350.041.8891373
TTACGAC257950.041.3069955
GTTACGA266650.039.9592674
GGAGTCT305950.039.7338071
ACTCCTG669100.039.4886675
GCTTGTA698600.038.8925367
ATCGAAG1097150.038.846586
CCTGGCC575800.038.8353465
GTCCAAT116050.037.730851
TCTGGAG398700.036.8367545
CGAAGGA1166400.036.6078838
ACATCGA1120000.036.305644
TGGCCCG615100.036.2834787
Produced by FastQC (version 0.11.2)