FastQCFastQC Report
Wed 20 Jan 2021
HM5GVAFX2_n02_ncm12-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM5GVAFX2_n02_ncm12-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8390140
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2276452.7132443558748722Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG523640.6241135428014313No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)