FastQCFastQC Report
Wed 20 Jan 2021
HM5GVAFX2_n02_ncm12-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM5GVAFX2_n02_ncm12-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6968103
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2537243.641220573232055Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG721141.034915815681829No Hit
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA149420.21443425850622475No Hit
TGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAATA83870.12036274435093741No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)