FastQCFastQC Report
Wed 20 Jan 2021
HM5GVAFX2_n02_ncm12-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM5GVAFX2_n02_ncm12-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5931622
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2716304.57935451719614Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG675071.138086681855317No Hit
GGTGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAA169270.2853688249183781No Hit
TGTTGTATTACGGGCTCGAGTAATACCGGAGTGTCTTGACAATCCTAATA106980.18035539014455068No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC82350.13883217777532014Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC69970.11796098942245478Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)