FastQCFastQC Report
Wed 20 Jan 2021
HM5GVAFX2_n02_ncm12-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM5GVAFX2_n02_ncm12-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889841
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1764602.5611621516374616Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG423780.6150795061888946No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)