FastQCFastQC Report
Wed 20 Jan 2021
HM5GVAFX2_n01_ncm12-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM5GVAFX2_n01_ncm12-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6968103
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG225290.32331611630884327TruSeq Adapter, Index 20 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGTTT195150.2800618762380522TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGGTTG192070.27564173491694943TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGTATG166830.23941953785700357TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGTTG160820.230794521837579TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGTCGTATG152110.21829470660809694TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGTTTG131810.18916195699173793TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGGATG120100.17235680930663624TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATG119310.17122307175998977TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGGTTT105310.15113152030043184TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTG97000.13920574939836566TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTT87570.12567265437953487TruSeq Adapter, Index 20 (97% over 41bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG74140.10639911608654465TruSeq Adapter, Index 20 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)