FastQCFastQC Report
Wed 20 Jan 2021
HM5GVAFX2_n01_ncm12-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM5GVAFX2_n01_ncm12-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5931622
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATG896531.5114415584809686TruSeq Adapter, Index 19 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGTATG279190.4706807008268565TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGTTT206980.34894334129855203TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGTTG171840.289701535263036TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTTTG137880.2324490670511371TruSeq Adapter, Index 19 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGTTTG121820.20537384209580448TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGGATG103480.17445481185416062TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGGTTG91800.15476373915937328TruSeq Adapter, Index 19 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA82140.13847814307789674TruSeq Adapter, Index 19 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATT81520.1374328977807419TruSeq Adapter, Index 19 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGGATG73030.1231197807277672TruSeq Adapter, Index 19 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGGTG59850.10089988876566983TruSeq Adapter, Index 19 (97% over 42bp)
GATGTTGTATCTCAAAATGAGATATGTCAGTATGACAATACGTCACCCTG59370.10009066660013062No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)