FastQCFastQC Report
Sat 18 Dec 2021
HM57KDRXY_n02_3_SOR_DMSO_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM57KDRXY_n02_3_SOR_DMSO_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30738239
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT771010.25083089502947775No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA613960.19973818278919622No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC590000.19194333156170723No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA531380.17287262292416947No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC525110.17083281836672556No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA524230.17054653000778608No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT516980.16818790432334135No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC514940.1675242358548907No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT505430.16443036961226049No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT479070.15585473195130012No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA460870.1499337681641424No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT453650.14758490231011606No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA452490.14720752220060493No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT415260.13509557265138059No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATCAGTACGACCTG400810.13039458766652182No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGG399380.12992936908324512No Hit
TCAGAGCACTGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCAT397750.12939908496384583No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC389770.12680297007255362No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTA388890.1265166817136141No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT388120.12626617939954204No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA386220.12564805680637725No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT384680.1251470521782331No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACT375270.12208571870366419No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT372810.12128541260935605No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG365380.11886822794240098No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG362080.1177946465963779No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTC352530.11468776724652315No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT346710.1127943601453551No Hit
CCTTTATAGTTTCTTTACTTATTCAATGAAGCGGAGCTGGAATTCATTTT341760.11118398812632044No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA336430.10944999158865282No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATA333140.10837966351943584No Hit
ATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATC332780.10826254555441513No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCC329610.10723125680687172No Hit
GTATAGGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCG328360.1068245972061054No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA325470.10588440020913364No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA321480.10458634276348752No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG320040.10411787090340473No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA318020.10346070898856632No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG314640.10236110142809417No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT309370.10064662455126333No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)