FastQCFastQC Report
Sat 18 Dec 2021
HM57KDRXY_n02_3_SC_RAPA_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM57KDRXY_n02_3_SC_RAPA_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25869963
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT636890.24618898759151686No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA527130.2037614046838799No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT512890.19825695150781625No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC501920.1940165125091211No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA466750.18042159550054246No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT465720.1800234503621053No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA455320.1760033441099239No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC442060.17087770863839272No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC430480.16640147494606003No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG405210.15663338985061556No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT404080.15619658984436893No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA401750.15529593142440906No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA400940.15498282699515264No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATCAGTACGACCTG387900.1499422322328022No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT379730.14678412953277126No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGG372740.14408215427289167No Hit
TCAGAGCACTGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCAT370160.14308485868340823No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT340650.1316778071928437No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTA339800.1313492408164635No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT332340.12846558767787955No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATA328550.12700056818790192No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC324960.1256128584335432No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTC318060.1229456725546921No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT317980.12291474866044454No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT314380.12152317341930485No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT313950.12135695748772429No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA311210.12029781410974573No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT310260.1199305928655561No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA306700.11855447957154017No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG306020.118291626470436No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG303950.11749147070678069No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG289930.11207205823989776No Hit
GTATAGGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCG288480.11151156265666093No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACT287930.11129896088370904No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA286980.11093173963951938No Hit
CCTTTATAGTTTCTTTACTTATTCAATGAAGCGGAGCTGGAATTCATTTT278830.10778136791305035No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC275240.10639365815869162No Hit
ATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATC273690.10579450770764535No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC270630.1046116687526766No Hit
TTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGTAT260830.10082349170735189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)