FastQCFastQC Report
Sat 18 Dec 2021
HM57KDRXY_n01_3_SOR_RAPA_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM57KDRXY_n01_3_SOR_RAPA_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16068364
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT446810.2780681343788328No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA377070.2346660805045243No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT359630.22381245533148242No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT334390.20810457119343329No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA332070.20666074032179008No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT329760.2052231328590764No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC325330.20246616270330944No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC288360.1794582198909609No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA285360.17759119721211195No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC281160.1749773654617234No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA280940.1748404504652745No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA277850.17291741710606007No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATA277110.17245688484527735No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG272930.16985549991274781No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT272290.16945720174126003No Hit
TCAGAGCACTGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCAT271880.16920204197515068No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT266720.1659907629675305No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA266260.16570448615677366No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTC261680.16285416486706425No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATCAGTACGACCTG260800.16230650488126855No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC250480.1558839468660282No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG243190.15134708175642522No Hit
GTATAGGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCG238540.14845319660420936No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT237240.1476441534433748No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA234210.1457584605377374No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT230010.14314462878734885No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC224840.13992712637079918No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA223830.13929856206892002No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTA220450.1371950498507502No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGG220230.1370581348543013No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACT219840.13681542190605092No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCC219770.13677185804354444No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT218390.13591302761127394No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA212690.13236568452146094No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG212610.13231589725002496No Hit
GTTGTAGACGGCCTTGGTAGGTCTCTTGTAGACCGTCGCTTGCTACAATT204490.1272624891992738No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT198830.12374003974517879No Hit
TACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAG186980.11636530016372545No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT185110.1152015226939096No Hit
GTCTAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGTCAGGGCATCA184840.1150334906528132No Hit
CTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTCGGACTCT184180.11462274566346642No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT182180.11337806387756713No Hit
GAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTA181120.1127183825310405No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC179360.11162306255944911No Hit
ATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATC176210.10966268874665773No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT174840.1088100817233167No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCATCTTCGATCCCCTAACT174790.10877896467866921No Hit
ACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAA174580.1086482730911498No Hit
GTGTAACAACTCACCGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCT174570.1086420496822203No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT173530.10799481515355266No Hit
GTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAA171620.10680614404801883No Hit
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAT171130.1065011970104735No Hit
CCTTTATAGTTTCTTTACTTATTCAATGAAGCGGAGCTGGAATTCATTTT171060.10645763314796704No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA170470.10609045202112674No Hit
GATTAGAGGTTCCGGGGTCGAAATGACCTTGACCTATTCTCAAACTTTAA168240.10470263182984901No Hit
CTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAACCTTT167840.10445369547266915No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT167540.10426699320478425No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC167400.1041798654797713No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC166870.10385002480650798No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGA166760.10378156730828353No Hit
GAGTATAGGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATT166190.10342683299930223No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA165670.10310321573496842No Hit
GTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAAT165540.10302231141888496No Hit
GGATAGCAGAAGCTCGTATCAGTTTTATGAGGTAAAGCGAATGATTAGAG163720.1018896509937166No Hit
AACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGAC160750.10004129854165612No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)