FastQCFastQC Report
Sat 18 Dec 2021
HM57KDRXY_n01_2_SOR_RAPA_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM57KDRXY_n01_2_SOR_RAPA_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31896714
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT911610.28580060002419055No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT891310.2794363080786315No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA772720.24225692966366377No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA750050.2351496144712587No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT745120.2336040007130515No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT696410.2183328351628948No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT682100.21384647960915348No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC628620.1970798622077497No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC593800.18616337720556417No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA566170.1775010428973969No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA559690.175469485665514No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC548730.17203339503874912No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA535880.1680047668860184No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC525540.16476305364872382No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA520970.16333030418117678No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGG514770.161386530286474No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA506010.15864016588041013No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA495200.1552511020414203No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT488490.15314743706828232No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT483850.15169274176644026No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT481580.15098106971144426No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT468780.14696811715463856No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA466760.14633482307926765No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT466370.1462125534310525No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCATCTTCGATCCCCTAACT460700.14443494085315497No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT452840.14197073717374148No Hit
CTCGTAAGGTGCCGAGTGGGTCATTAAAAAAACACCACCCGATCCCTAGT449790.14101452582231513No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG449370.14088285081654492No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT443240.1389610227561372No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT429660.13470353090290116No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA419620.1315558712411567No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA414860.13006355450909457No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA410710.12876248004731775No Hit
ATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATC403590.12653027518759458No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATCAGTACGACCTG403090.12637351922834433No Hit
TTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGTAT400490.12555838824024318No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACT399610.1252824977519628No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG394690.1237400191129406No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCC394590.12370866792109055No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTC391750.1228182940725493No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG386980.12132284222130217No Hit
GTATAGGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCG386060.12103441125628177No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC386000.12101560054117173No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC382850.12002803799789533No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT381280.11953582428584965No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT380620.11932890641963934No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT377410.11832253316125291No Hit
CTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTCGGACTCT374410.11738199740575159No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGA372130.11666719023157056No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA368970.11567649256910915No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT367500.1152156300489135No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG365240.11450709311310249No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC361250.11325618055828572No Hit
GAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTA361210.11324364008154571No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC359080.11257585969513975No Hit
GTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAAT355250.11137510904728305No Hit
TTCTAGGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATT353850.11093619236138243No Hit
GTGCCGAGTGGGTCATTAAAAAAACACCACCCGATCCCTAGTCGGCATAG350970.11003327803610116No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTA349690.10963198278042058No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT349060.10943447027176531No Hit
AACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGAC343990.10784496484496804No Hit
CCTTTATAGTTTCTTTACTTATTCAATGAAGCGGAGCTGGAATTCATTTT337240.10572875939509005No Hit
TCAGAGCACTGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCAT337020.10565978677301993No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC336050.1053556802120745No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA334880.104988871267429No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA333630.10459698136930343No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTA328360.10294477355880609No Hit
GTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTT324700.10179731993709445No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA320990.10063419071945781No Hit
CAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTGTGGGTGGTGG319730.10023916570214725No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT319420.10014197700741212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)