FastQCFastQC Report
Sat 18 Dec 2021
HM57KDRXY_n01_2_SOR_DMSO_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM57KDRXY_n01_2_SOR_DMSO_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15856753
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT646410.4076559684066467No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT428040.2699417718116692No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA394500.24878990042917362No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA377180.23786710936343652No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA351820.22187392336880069No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC312550.19710844963026164No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA306680.19340655681525717No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT288070.18167023223480874No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA257650.16248597679487095No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA257470.16237246049049261No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT245280.1546848840995379No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAAT242880.1531713333744935No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTC241130.15206770263748196No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG237280.14963971501605655No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC223170.14074129804506635No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA220140.1388304402546978No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT217270.13702048584599888No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT216350.13644029140139852No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC216020.1362321781767049No Hit
GTATAGGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCG214050.13498980528989762No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA208750.13164738077209123No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACT206860.13045545957611876No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG204760.1291311026917049No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCC200530.12646346953881415No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT199940.12609138831890743No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT199900.12606616247349No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA198160.12496883819783282No Hit
TCAGAGCACTGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCAT197430.12450846651896515No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC195560.12332915824570137No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATA195040.12300122225527509No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC189230.11933716820839675No Hit
GTCTAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGTCAGGGCATCA187800.11843534423472447No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT184000.11603888892007083No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATCAGTACGACCTG178660.11267123855684703No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC170060.10724768179210459No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG169170.1066864067315673No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC168650.106358470741141No Hit
TACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAG167310.1055134049196579No Hit
GTGTAACAACTCACCGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCT164790.10392417665836128No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTA161960.10213944809507976No Hit
GTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAAT161450.1018178185660078No Hit
AACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGAC159870.10082139767202024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)