FastQCFastQC Report
Tue 11 Jun 2019
HM2GTBGXB_n02_Christsos_RAM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM2GTBGXB_n02_Christsos_RAM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556462028
Sequences flagged as poor quality0
Sequence length92
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGG37287380.670079504508437No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37019510.6652656989561918No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAAACGAGGAAAGGCTTAC36133330.6493404434057808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGTCGTGCCTCCGGC22389540.4023552169493226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATGCATTGGATGGATGCC18113260.3255075654506295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACGCTCTGTATACATTAGCA14685570.2639096517112215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGCTGGCGACGCATCATTC13314390.2392686172649322No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCTGGCGACGCATCATTCA10215040.18357119598464317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGTGCTGGCGACGCATCATT9569880.1719772332785302No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGCCGGGTCGTGCCTCCGG8059270.14483054717976193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCGGGTCGATCCGGGCGGA7730260.13891801436629203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACGCATCATTCAAATT7116430.12788707300617463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCCCCGGGCGTCGAACAGTC6389480.11482328853533201No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGCGGGTCGATCCGGGCGG6334810.11384083156164612No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGGCGACGCATCATTCAA6312710.11344367957484423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGCTTTCCCCGGGCGTC6173870.11094863062246539No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTCGCAGTGACCAGGC5900090.10602861836243747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT107203950.083.084891
AGCAGTG120938500.073.57962
GCAGTGG125146700.071.0827263
CAGTGGT125359600.070.857934
AGTGGTA126090950.070.496785
GGTATCA126583500.070.163148
GTGGTAT126812500.070.0343256
TGGTATC126878800.069.952897
GTATCAA127404950.069.7498259
CCCTGCG892600.054.2270486
GTAGTAA7537800.042.73688586
ATCAACG126595650.039.78512210-11
CAACGCA126927100.039.67422512-13
ACGCAGA127201900.039.58123414-15
AGTACAT126093500.039.49005520-21
GCAGAGT127185700.039.44124216-17
AGAGTAC127110650.039.3605718-19
TACATGG125986850.039.3467822-23
CATGGGG60953900.039.09589824-25
CATGGGT22451200.037.04084824-25
Produced by FastQC (version 0.11.5)