FastQCFastQC Report
Tue 11 Jun 2019
HM2GTBGXB_n01_Christsos_RAM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHM2GTBGXB_n01_Christsos_RAM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556462028
Sequences flagged as poor quality0
Sequence length29
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA1407500.019.5396461
ACCCGAG1249250.018.1145673
TCGGAGG1202250.017.8378434
AACGGGA1067350.017.6990241
GACCCGA1359400.017.6105862
CGGAGGT1129500.017.5452945
GATCGGA1083400.016.9529862
ACTACGA1811250.016.8237821
GAGACCC1244750.016.7290821
TCTGCGA1052950.016.6692168
ATGGGAG862950.016.5940531
ATCGGAG1582700.016.4968153
CCCGAAG1388750.016.4083181
CTACGAC1061400.016.3324872
GGGTAGA1345350.016.3254571
CCGAAAG1484950.016.2685383
GAATAGA1511800.016.163121
GGAGATG1549250.016.1250861
ATACCGA1045700.016.0931451
GACCTGC1287450.016.08570110
Produced by FastQC (version 0.11.5)