FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21346684
Sequences flagged as poor quality0
Sequence length49
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12775875.984943609977082No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAA1011050.4736332818717886No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT601480.28176741642870623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGC662100.043.3752251
CATAGCT678850.042.800052
ATAGCTC690750.042.5647623
TAGCTCT686700.042.294634
CTAACGG119350.041.9125146
GTCCGAC205950.041.73312814
GCATTCT227800.041.567141
TCTAACG121150.041.5346645
TGTACCC323100.041.46676610
TAACGGC119150.041.448747
GACGTAT116250.041.4462135
TTGTCCG209050.041.42812712
AGTTGTA332100.041.413467
TGTCCGA208200.041.40413313
TGACGTA115400.041.391324
GTACCCC324750.041.2190311
TCCGACA211550.041.206115
CGACAAT219500.041.1849817
GTTGTAC329950.041.1849488
ACCCGTG148300.041.154314
Produced by FastQC (version 0.11.5)