FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n02_cDNA5n_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n02_cDNA5n_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82452182
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8601271.0431828232271645No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT7784830.9441630058983763No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1350650.16381009783343273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1080500.034.393661
ATCAACG1203650.030.59371611
AGCAGTG1220550.030.41962
TCAACGC1222550.030.11694112
CAACGCA1240600.029.71872313
TGGTATC1242600.029.669347
GTATCAA1249000.029.5139059
TATCAAC1254950.029.42013510
GGTATCA1269250.029.0770458
AACGCAG1281300.028.88678214
ACGCAGA1288550.028.66212315
AGAGTAC1285600.028.524819
AGTGGTA1302100.028.404295
GTGGTAT1319950.027.9990146
CAGTGGT1335400.027.7676264
CGCAGAG1338150.027.6694816
CAGAGTA1364900.027.03720918
GCAGAGT1385100.026.64898117
GTACTTT1363800.026.54428922
GAGTACT1375750.026.51576220
Produced by FastQC (version 0.11.5)