FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n02_cDNA5n_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n02_cDNA5n_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78282268
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT7507220.9589936765756455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6848940.8749031134355995No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1288930.16465159134122176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1032200.034.4768751
AGCAGTG1154650.030.7556382
ATCAACG1160200.030.49048811
TCAACGC1177100.030.01950512
CAACGCA1195550.029.57433113
GTATCAA1199200.029.5436849
TGGTATC1196650.029.5419657
TATCAAC1210050.029.29830410
GGTATCA1218900.029.0170358
AACGCAG1237150.028.64395114
AGTGGTA1245850.028.4150475
AGAGTAC1239000.028.33094619
ACGCAGA1249200.028.3139915
GTGGTAT1265700.027.9693746
CAGTGGT1277200.027.7623444
CGCAGAG1281800.027.58786216
CAGAGTA1312150.026.91677718
GCAGAGT1327350.026.53514517
GTACTTT1314000.026.48015422
GAGTACT1323900.026.39004720
Produced by FastQC (version 0.11.5)