FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n02_cDNA5n_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n02_cDNA5n_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141832769
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13223500.9323303841018572No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT12764640.8999781989731864No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2222670.15671061177688775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1771300.034.1739961
ATCAACG1984000.030.34776911
AGCAGTG1995750.030.3071292
TCAACGC2014000.029.87089312
CAACGCA2047050.029.42343313
TGGTATC2049400.029.362457
GTATCAA2054650.029.335429
TATCAAC2073450.029.11806510
GGTATCA2088200.028.857138
AACGCAG2118100.028.53053714
AGTGGTA2139050.028.2373165
ACGCAGA2136300.028.21069315
AGAGTAC2121950.028.20399319
GTGGTAT2169900.027.8021836
CAGTGGT2194900.027.505594
CGCAGAG2204750.027.38243516
CAGAGTA2261000.026.6407918
GCAGAGT2286850.026.33555217
GTACTTT2249300.026.27344922
GAGTACT2277100.026.1245920
Produced by FastQC (version 0.11.5)