FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n02_cDNA5n_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n02_cDNA5n_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78516757
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT7568870.9639814848695292No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6871060.8751074627292617No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1300190.16559395085561163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1031900.034.4597241
AGCAGTG1148850.030.899562
ATCAACG1163850.030.39286411
TCAACGC1177850.030.00027812
CAACGCA1196300.029.54675313
TGGTATC1199000.029.5234387
GTATCAA1209750.029.285959
TATCAAC1218600.029.10848810
GGTATCA1226050.028.9002918
AACGCAG1237350.028.64620214
ACGCAGA1246500.028.41989515
AGTGGTA1251050.028.3792445
AGAGTAC1247050.028.18268219
GTGGTAT1274600.027.802636
CAGTGGT1280950.027.7245864
CGCAGAG1279700.027.69345516
CAGAGTA1317100.026.86650318
GCAGAGT1331250.026.52708617
GTACTTT1314050.026.45465722
GAGTACT1323100.026.4223120
Produced by FastQC (version 0.11.5)