FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n02_HTO5n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n02_HTO5n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27597023
Sequences flagged as poor quality0
Sequence length49
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1809790.6557917497115541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA4363150.042.818331
ACTGTCT5046750.042.7287861
CTGTCTA5047500.042.7226032
CGATAAT4375550.042.7112962
GTCTAAC5045500.042.706844
GGCTGAG7439250.042.701953
GAGGCTG7455100.042.6951261
GCTGAGC7430100.042.6838384
CTAACGG5037950.042.671656
TGTCTAA5058500.042.6442263
CATGTTA2422400.042.638312
CGTATTC4842950.042.631887
TAACGGC5028700.042.615837
GTATTCC4834100.042.6077968
TAATGCG4355800.042.5897675
TGAGCTA7463900.042.5892686
GAGCTAC7445650.042.584447
ACGTATT4892750.042.581186
GTGACGT4896000.042.576813
TATTCCC4832300.042.566949