FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n02_GO5n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n02_GO5n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48736394
Sequences flagged as poor quality0
Sequence length49
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCATATAAGAAA840059417.236798438554974No Hit
GCATAGCTCTTAAACTTGCGACGCTTAGCCTCCGTCCCCATATAAGAAA17464803.5835232290677888No Hit
GCATAGCTCTTAAACTGTCATAGGGCACCAGGTCTCCCCATATAAGAAA13092252.6863394940544842No Hit
GCATAGCTCTTAAACCGTCTTGGGATCTGGCTCCTCCCCATATAAGAAA12926992.652430542973696No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCATATAAGAAA11719022.40457264852217No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCATATAAGAAA10525712.1597227730882183No Hit
GCATAGCTCTTAAACCACCTCTCAATGCCGTGGTTCCCCATATAAGAAA9807682.0123934487233504No Hit
GCATAGCTCTTAAACTGGGCAAGCTCTACGGCAACCCCCATATAAGAAA9721101.9946284905690805No Hit
GCATAGCTCTTAAACCCTGCTAATGTTGAGCCTGGCCCCATATAAGAAA9700321.9903647364636785No Hit
GCATAGCTCTTAAACTGTATGTGCTCATTGACCGTCCCCATATAAGAAA9006181.84793729302172No Hit
GCATAGCTCTTAAACCAATGTTTTCTGGAGGCCCGCCCCATATAAGAAA7972761.6358945226846289No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCATATAAGAAA6737211.3823776129190026No Hit
GCATAGCTCTTAAACTTGATTTGTCAAGGCAGTGCCCCCATATAAGAAA6551441.3442603078102169No Hit
GCATAGCTCTTAAACCCGGAGTATCCGTTCAAGCCCCCCATATAAGAAA6440981.3215955205877563No Hit
GCATAGCTCTTAAACGATGTTAATGATTTTAGGTCCCCCATATAAGAAA6439641.321320572055454No Hit
GCATAGCTCTTAAACCACTTTAGATTCATTGATGCCCCCATATAAGAAA6149031.2616916220761019No Hit
GCATAGCTCTTAAACCACATGACAGGACTTCAGCTCCCCATATAAGAAA5110071.0485121242248656No Hit
GCATAGCTCTTAAACCCACATAAAGTACATTGTGACCCCATATAAGAAA5064471.0391556667077175No Hit
GCATAGCTCTTAAACCTGTCACGGACTCTGCACCCCCCCATATAAGAAA5061561.0385585769845835No Hit
GCATAGCTCTTAAACCATGTCTCGCCCCGATATGGCCCCATATAAGAAA5055811.0373787605213467No Hit
GCATAGCTCTTAAACCGCCGTTAAGCGGAAACGATCCCCATATAAGAAA4827460.9905246580204518No Hit
GCATAGCTCTTAAACCTCCAAAGCTCCAAACATCTCCCCATATAAGAAA4792740.9834006184372196No Hit
GCATAGCTCTTAAACCATTCCTTGGATTACAGCTGCCCCATATAAGAAA4743000.9731946930665407No Hit
GCATAGCTCTTAAACCAGCAATTTCTGGCTGGTTGCCCCATATAAGAAA4540320.9316077016284792No Hit
GCATAGCTCTTAAACCCCAGTACACCTTGCACTTGCCCCATATAAGAAA4359540.894514272024311No Hit
GCATAGCTCTTAAACCCTTGCAGGACGGCAGAGCTCCCCATATAAGAAA4311890.8847371842898348No Hit
GCATAGCTCTTAAACCTTCATTTCTCAGCAGGGTGCCCCATATAAGAAA4197070.861177788410033No Hit
GCATAGCTCTTAAACCTGCATCAGATCTCTGCCATCCCCATATAAGAAA4182630.8582149101962694No Hit
GCATAGCTCTTAAACGAAGAAGCTGTTTCAGCTGTCCCCATATAAGAAA3931160.8066169195858027No Hit
GCATAGCTCTTAAACCCATTACCAGTTGCTCACTGCCCCATATAAGAAA3827760.7854007417947253No Hit
GCATAGCTCTTAAACTTGAACGGGCCGCGGAAGCGCCCCATATAAGAAA3767040.7729418799429437No Hit
GCATAGCTCTTAAACCACTGAATTCTCAGTACTGTCCCCATATAAGAAA3729320.7652022839441096No Hit
GCATAGCTCTTAAACCAGGTATGGGGGAGGCTTCTCCCCATATAAGAAA3708060.7608400408122111No Hit
GCATAGCTCTTAAACACGAGAAGATGTCTCGTGCCCCCCATATAAGAAA3542300.7268284969954897No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3132760.642796838846961No Hit
GCATAGCTCTTAAACCTTCTTGCGTCTCCGGCCGGCCCCATATAAGAAA3101270.6363355483378602No Hit
GCATAGCTCTTAAACCTCTGCCTGATGACCACAGCCCCCATATAAGAAA3011950.6180083819906742No Hit
GCATAGCTCTTAAACCAGGACAGCAACTTCTGCCCCCCCATATAAGAAA2976690.6107735422526337No Hit
GCATAGCTCTTAAACCCTTCGTGTACGGTTGCACACCCCATATAAGAAA2943280.6039182956375476No Hit
GCATAGCTCTTAAACCAGAAAACTTGCTCTCTGTCCCCCATATAAGAAA2817770.5781654670634845No Hit
GCATAGCTCTTAAACTGGTGTGCGCGTCGTACACTCCCCATATAAGAAA2802330.5749974033778535No Hit
GCATAGCTCTTAAACTGTCTATTCCATCTACGTCCCCCCATATAAGAAA2714380.5569513411271256No Hit
GCATAGCTCTTAAACCCTCGATATCTGGCAGGGAGCCCCATATAAGAAA2382450.4888441274502172No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCCCATATAAGAA2376820.48768893324360435No Hit
GCATAGCTCTTAAACCTTTGTTGTGGTGACAGGTCCCCCATATAAGAAA2376420.48760685905485746No Hit
GCATAGCTCTTAAACGTATGACCCTCTACAGGACACCCCATATAAGAAA2137020.438485457089829No Hit
GCATAGCTCTTAAACCCTGCTGTGGGATTACGTGTCCCCATATAAGAAA2069480.42462723031991245No Hit
GCATAGCTCTTAAACCGTGCTATTGCTCAGGGCCACCCCATATAAGAAA2065440.42379828101356865No Hit
GCATAGCTCTTAAACCCGTGGTGACGTCCGGAGGGCCCCATATAAGAAA2026860.41588222550892867No Hit
GCATAGCTCTTAAACTTTTTGTCGGCATCTTGAAGCCCCATATAAGAAA2006820.41177030865270825No Hit
GCATAGCTCTTAAACCCAGCATGTTGTACCAAAGGCCCCATATAAGAAA1817070.3728363653658906No Hit
GCATAGCTCTTAAACCCACGGCCTTTTTTTGTTATCCCCATATAAGAAA1758310.36077966703896885No Hit
GCATAGCTCTTAAACCCCCAGTGTTGTCCTGTAGACCCCATATAAGAAA1698390.348484953564681No Hit
GCATAGCTCTTAAACTGGAGGAGACAATACCCTCACCCCATATAAGAAA1673810.3434414946661832No Hit
GCATAGCTCTTAAACCCTTTCTGATATCCAGTTTACCCCATATAAGAAA1544360.3168802353329629No Hit
GCATAGCTCTTAAACCTTGTCCTTGAACTCTATTCCCCCATATAAGAAA1444900.29647248830104256No Hit
GCATAGCTCTTAAACCTGTCTATGGAGACTTTAGCCCCCATATAAGAAA1403650.28800858758651693No Hit
GCATAGCTCTTAAACCGTGGTGAATGGTTTCAGGTCCCCATATAAGAAA1286250.2639198131892975No Hit
GCATAGCTCTTAAACCCAGATGAATTCTGAACTGTCCCCATATAAGAAA1227590.2518836334095625No Hit
GCATAGCTCTTAAACCCAGAAGGGTCCACGTATCCCCCCCATATAAGAA1195300.24525819452296777No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCATATAAGAAAA1137530.23340462981319465No Hit
GCATAGCTCTTAAACCGTCGCAGTAGAAATACGGCCCCCATATAAGAAA1116740.22913882385307374No Hit
GCATAGCTCTTAAACCGTGCACAGCGAGTTCGAGACCCCATATAAGAAA1110440.22784615538030983No Hit
GCATAGCTCTTAAACCGTGGTCACTAAGGCACCTGCCCCATATAAGAAA1097330.22515617384412973No Hit
GCATAGCTCTTAAACCCTGGCAGAAACTTCAGCTGCCCCATATAAGAAA1043690.21415002513316844No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCATATAAGAAAC1034730.21231156330523757No Hit
GCATAGCTCTTAAACCGTCATCGTTGAGGTGTTTACCCCATATAAGAAA1021050.2095046260500931No Hit
GCATAGCTCTTAAACCGCAGTGGACCTGCAAAATCCCCCATATAAGAAA984140.20193123028347154No Hit
GCATAGCTCTTAAACCACGTGTCTACACTTAGCCTCCCCATATAAGAAA964690.19794037285565277No Hit
GCATAGCTCTTAAACGTAGAGAGCGGCGCGCCTACCCCCATATAAGAAA961890.19736585353442437No Hit
GCATAGCTCTTAAACCTCGGGAGAAAGAGGAGCGTCCCCATATAAGAAA932000.19123285978031118No Hit
GCATAGCTCTTAAACCGCAACTCTTATAAATGTGGCCCCATATAAGAAA910200.18675981649360435No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCATATAAGAAAT905620.18582006703245219No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCCCATATAAGAAAG884330.18145166833639764No Hit
GCATAGCTCTTAAACCCGTAGTGGGGCTGTACACCCCCCATATAAGAAA845610.1735068868656963No Hit
GCATAGCTCTTAAACTGGGGGTATCGAGGACGTGCCCCCATATAAGAAA828830.17006387464776324No Hit
GCATAGCTCTTAAACCACCTTCAAGCAACGCCGCACCCCATATAAGAAA796990.16353076922350882No Hit
GCATAGCTCTTAAACTGGCTTTGCTTTTTTTATTCCCCCATATAAGAAA796680.16346716172722994No Hit
GCATAGCTCTTAAACCTGGTCTATCTCTATCCTGACCCCATATAAGAAA726890.14914726764561204No Hit
GCATAGCTCTTAAACCTGTAGGAGCGGCGTTAGTACCCCATATAAGAAA726130.1489913266869929No Hit
GCATAGCTCTTAAACGGACCATCTTTCCCGGAGTCCCCCATATAAGAAA710750.14583557412967402No Hit
GCATAGCTCTTAAACCACAAGTTTTGATTCGGACACCCCCATATAAGAA676840.1388777347786543No Hit
GCATAGCTCTTAAACCACGTGAGTATTCCAGAACACCCCATATAAGAAA624420.12812191234337114No Hit
GCATAGCTCTTAAACTTCTGATATCCAGTTTAGGTCCCCATATAAGAAA601740.12346830584142109No Hit
GCATAGCTCTTAAACGACTTTCCTTTTTTGTCGGCCCCCATATAAGAAA586890.1204213015841919No Hit
GCATAGCTCTTAAACCCGTGTAGCGCAGCTGCTTCCGCCCATATAAGAA559530.11480742707390293No Hit
GCATAGCTCTTAAACCCTTCTCTCGCACTTCTATCCCCCATATAAGAAA548700.11258526841358021No Hit
GCATAGCTCTTAAACCAGGACAGCAACTTCTGCCCCCCCCATATAAGAA545170.1118609636978887No Hit
GCATAGCTCTTAAACTGACAGAGGAGAAGTTCACACCCCCATATAAGAA522920.10729558694884155No Hit
GCATAGCTCTTGGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA497180.1020141129029776Illumina Single End PCR Primer 1 (100% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGC46262350.042.9964261
CATAGCT46284100.042.959292
GCATTCT619750.042.9573141
ATTCTGT618850.042.9329643
ATAGCTC46345650.042.9305533
TAGCTCT46334650.042.9010854
AGCTCTT46345700.042.8707165
GCTCTTA46077200.042.846336
CATTCTG619450.042.827552
CTCTTAA46120200.042.820827
TTCTGTC619650.042.807934
GAATAGC184150.042.802621
TCTTAAA46152650.042.7834478
GTTTTCT968700.042.74360720
CTTAAAC46156350.042.7421239
TTTCTGG1486600.042.73572522
TTTTCTG968450.042.73549321
TACCAGT471600.042.7160720
TTAAACT9451750.042.71153610
TAAACTG5771800.042.70786311
Produced by FastQC (version 0.11.5)