FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n02_BLG5n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n02_BLG5n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36164355
Sequences flagged as poor quality0
Sequence length49
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1076440.2976522047745632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA5130800.042.56593311
GTCCTAC5124900.042.5405212
TCAACGC3553700.042.52601211
TCCTACC5090500.042.5181313
TTGTCCG14653650.042.48171612
GTCTTTG11105000.042.476311
AACGCTT3553650.042.4747213
TGTCCGA14651350.042.47471213
CAACGCT3553100.042.45697412
GTCCGAC14643300.042.44488514
TCCGACA14675950.042.4370315
AGTCTTT3489850.042.43658410
ATGTCCT1238200.042.41664510
CTGTCCT1340350.042.40149310
CCGACAA14694000.042.38171416
CGTCTTT2125250.042.3744410
TCTTTGT11123050.042.36387312
GGTCTTT3905200.042.36017210
CGACAAT14696350.042.3334217
TTTGTCA11101750.042.33210414