FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n02_ADT5n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n02_ADT5n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28247895
Sequences flagged as poor quality0
Sequence length49
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2109640.7468308700524411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCT7544250.042.938691
TTAACCA11436550.042.8912241
TAACCAG11459700.042.860282
CATTCTG7552850.042.8477822
ACCAGTT11448550.042.7793054
CCAGTTG11449700.042.7720835
TTCTGTC7568700.042.7627144
ATTCTGT7587950.042.7615283
GTTGTAC11436350.042.7536628
TCTGTCA7559300.042.7426835
GTACCCC11350750.042.7393311
TGATCTA4044300.042.7277452
TACCCCC11292850.042.72388512
TTGTACC11384900.042.7166529
AGTTGTA11508750.042.7102857
CTGTCAC7550100.042.7087756
GATCTAA4054800.042.7023933
AACCAGT11497550.042.684233
CAGTTGT11491250.042.675996
TGTCACC7523950.042.674067