FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21346684
Sequences flagged as poor quality0
Sequence length26
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG728840.341430078788818No Hit
CGTATGCCGTCTTCTGCTTGAAAAAA476100.2230322985996326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT38800.018.0859131
TTGAAAA57700.017.6036218
GCGCGAT16900.017.1080591
CCGGGAT23700.016.6471231
CCTTACG29750.016.6362291
GGGAATG26350.016.4968971
CTTGAAA61700.016.34830717
GGGACCT18550.016.2898671
GGAACTT30500.016.0625671
ATGCCGT63450.016.0284484
ATCGAGT22200.015.9178581
TGCGTGG25050.015.7499721
CTGATCC28000.015.6681741
TCGAATG19800.015.652358
CGCTTCA19650.015.5312351
CGATTGA24450.015.5205781
GTACTCC29000.015.5086861
TGCGGGT17350.015.3913641
TGACTAG23200.015.3615611
TACGGGC24000.015.3096341
Produced by FastQC (version 0.11.5)