FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n01_cDNA5n_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n01_cDNA5n_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82452182
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT255500.017.2721751
GACTAAC232550.017.0287511
ACTGAAC213650.016.976521
CAATGGA278150.016.9622278
GCGCGAT179550.016.8812471
GGAACTT261200.016.7765711
CCTTACG279150.016.7080171
CGGACTG198950.016.6676581
ATCGAGT219500.016.6288551
CGATTGA234700.016.5489371
TTTATGC281400.016.464261
GATGCTA208350.016.4244371
GTGATAA196650.016.2828878
TGATTTC249650.016.2629181
CTCGAAA191200.016.2553791
TGACTAG214900.016.2123411
CCGGGAT180650.016.1529311
CAACTAG328100.016.1324251
GGATTAC233800.016.1164881
GAATGAA231400.016.0805281
Produced by FastQC (version 0.11.5)