FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n01_cDNA5n_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n01_cDNA5n_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78282268
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT239800.017.3270681
GACTAAC220000.017.136491
ACTGAAC205200.017.0712781
CAATGGA275550.016.9808628
GCGCGAT172300.016.8370381
GGAACTT247350.016.7617991
CCTTACG257750.016.5432851
ATCGAGT206550.016.4755521
CGGACTG193150.016.4588341
GATGCTA204150.016.4586071
TTTATGC261500.016.3404661
CGATTGA225150.016.3358971
CTCGAAA180600.016.317951
TGACTAG201450.016.3019331
GTGATAA194550.016.2994178
ACTGATG178550.016.2588711
GAATGAA216500.016.221831
TGAGCCG170900.016.1967261
CCACTAC277600.016.1240391
CAACTAG321050.016.103531
Produced by FastQC (version 0.11.5)