FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n01_cDNA5n_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n01_cDNA5n_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141832769
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT445650.017.3814531
GACTAAC391900.017.1039521
ACTGAAC359450.016.9676881
CAATGGA492400.016.850258
GCGCGAT317700.016.8241441
GGAACTT448850.016.6470831
CCTTACG461200.016.6154481
CGGACTG342200.016.5927311
ATCGAGT372100.016.5467221
GATGCTA363100.016.5134531
TTTATGC473250.016.4649661
CTCGAAA324400.016.3965191
CGATTGA419500.016.3958031
TGACTAG362850.016.340181
GTGATAA344800.016.3110288
CCGGGAT300800.016.306611
GGGACCT308550.016.2502961
GGATTAC397400.016.2230431
CAACTAG574050.016.1607061
GAATGAA392200.016.101571
Produced by FastQC (version 0.11.5)