FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n01_cDNA5n_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n01_cDNA5n_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78516757
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT245800.017.4777641
CAATGGA272000.017.0700118
ACTGAAC204100.016.9134121
GCGCGAT173250.016.9121131
GACTAAC221600.016.9089811
CGATTGA230750.016.5981851
GGAACTT243900.016.56841
CCTTACG263300.016.5288071
ATCGAGT206100.016.453311
GATGCTA199300.016.4426821
CGGACTG184050.016.4358831
GGGACCT164050.016.4158571
ACTGATG175650.016.3849871
TGACTAG200100.016.3769421
TTTATGC267350.016.30461
CTCGAAA178700.016.2676161
GAATGAA216050.016.2602561
TGATTTC240700.016.1945631
AAACCTG203200.016.171391
GTGATAA187500.016.1708168
Produced by FastQC (version 0.11.5)