FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n01_HTO5n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n01_HTO5n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27597023
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC1069500.019.587872
ATACCAC1071800.019.5723514
TACCACT1071650.019.5472015
CCACTTA1075400.019.5307257
GATACCA1075500.019.4953673
ACGATAC1128150.019.2866861
CTTACGA1103850.019.2528610
ACCGAGT519150.019.2378064
ACTTACG1106800.019.215139
CACTTAC1107500.019.1288078
GCGACAC479400.019.0911184
TTGCAGG532850.019.05250710
TTACCGA535050.018.962922
CTTACCG576250.018.9468781
TGCGACA479100.018.9378323
ATTCGAG355750.018.9095863
TTACGAA240150.018.90887811
CCGAGTT529300.018.8841155
ACCACTT1109850.018.881756
CGAGTTG526300.018.8588876