FastQCFastQC Report
Wed 6 Feb 2019
HLYLTBGX9_n01_GO5n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYLTBGX9_n01_GO5n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48736394
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAAGAGCTATGCTGGAATTCTCG1792780.36785241025423426No Hit
CCTAAAGAGCTATGCTGGAATTCTCG892540.18313624106042806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGGA49700.019.5780475
CTTTGGT50650.019.54616510
ACTAGGT126000.019.5159323
AGCTACA402150.019.4331073
GGTATTG219500.019.3712791
CCCGAGA7200.019.30535711
CGAAGCT87150.019.2891565
GCTACAA402550.019.2626764
CAGCTAC406900.019.201182
CCGTACA167200.019.1570329
GAAGCTA65200.019.126596
ACCCCCC14700.019.11525314
CGCGATG29000.019.103352
CAATGGA145650.019.0665268
CCATTGA16100.019.00578119
TCTATAA16100.018.9437717
CAAATTC292150.018.8670793
CTAAAAT20700.018.79182613
GCCGAGG13150.018.78294216
CAAGGAC100150.018.7820978
Produced by FastQC (version 0.11.5)