FastQCFastQC Report
Fri 8 Feb 2019
HLYKLBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84528870
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6557612077.5783705614425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7184660.8499652248989014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG30750.014.3372791
CCGACGC17800.014.170521145
CGACCCG4854.6735484E-513.373298145
GTATTAG46800.012.7733261
TATACTG60250.012.6824685
GTATAGA49100.012.4793581
GTATATA111850.012.2925421
CCGTATA10455.2204996E-1011.723852145
TCCGATC8503.9573024E-711.428371
TCTATAC48400.011.0532973
GTCCTAT28650.010.9542971
GTCCTAG28400.010.7876141
AACGCGC7351.6941376E-510.785569145
TAGACAG117650.010.6790275
CTAGGAC31400.010.64847853
GTGTATA74500.010.6318651
TATACAG64150.010.5370415
GTATTAT73100.010.4265991
GTATAGT41500.010.263291
GTCTTAG37950.010.2388561
Produced by FastQC (version 0.11.5)