FastQCFastQC Report
Fri 8 Feb 2019
HLYKLBGX9_n02_Sample_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20501312
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC19100.027.327581145
GCCGACC18000.017.720757145
GTATATA357500.014.2532971
GGATCGT38600.013.7126818
TATACTG179900.013.3819435
CCTATAC86200.013.2082713
GATCGTT42800.012.5370069
GTGTATA256300.012.0929851
GTCCTAG98350.012.0300521
GTATAGA129300.011.9573611
GTATTAG138550.011.8925121
CCGTACC29350.011.8595344
CTATACT138600.011.7720964
GTATAGG80800.011.7683131
TCCGTAC32150.011.7294033
GTCCTAA107050.011.6626171
GTATAAG129650.011.4212051
CTGCCGC60400.011.402202145
CTTACAC150150.011.1568143
GTATTAT211250.011.1327521
Produced by FastQC (version 0.11.5)