FastQCFastQC Report
Fri 8 Feb 2019
HLYKLBGX9_n02_Sample_72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22161887
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC16700.024.743586145
GTATTAG158450.015.2110791
GTATATA433650.014.3300781
TATACTG226600.013.5360075
TATACCG16950.013.2617885
GTATAAG151650.013.068761
CTATACT152850.012.4776484
TCTATAC149300.012.4349823
GTATAGG87250.012.314321
GGATCGT35450.012.2717898
GTATTAT251000.012.263271
GTCCTAG112050.012.1803611
GTATTAA217900.012.1604541
TATACAG211100.012.0223655
CTATACG19100.011.7698474
GTCCTAA118250.011.7259051
CCTATAC84800.011.6307583
GTCCTAT99650.011.5833441
GTATAGA150500.011.4803361
TAACCCG33600.011.437915
Produced by FastQC (version 0.11.5)