FastQCFastQC Report
Fri 8 Feb 2019
HLYKLBGX9_n02_Sample_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8413447
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGC27150.016.288109145
GCCGACC7352.1827873E-1115.781318145
GTATATA148300.015.3206391
TATACTG78800.015.1830395
GTATTAT88250.014.8057851
CTAGCGC4051.4450315E-414.3247673
CCGTATA11200.014.240173145
GTACTAG26050.013.9327211
TAGTCCG4703.2829113E-513.8849935
CGCCGTT4201.9732815E-413.808654145
GTATTAA76250.013.8039011
GTATTAC41600.013.6105281
TATACAG72900.013.527345
TAATACG10603.6379788E-1212.9980944
ATAATAC87000.012.836723
GTATTAG56050.012.8213361
TTATACT90650.012.7992394
GTGTATA103450.012.7706971
GTATAGA53400.012.6419891
GTATAAG54000.012.6359461
Produced by FastQC (version 0.11.5)