FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16342563
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC12400.028.646627145
TCGTACG2653.7786958E-621.8915314
GCCGACC14300.017.23622145
CCTATAC65900.015.2961223
TATACTG155900.014.2788235
GTATATA293200.013.3449091
GTATAGA107050.013.1554551
CCTACAC77000.013.0910963
GTATAGG63150.012.9896041
CTATACT111900.012.8959994
GTATTAG114100.012.7879581
GTGTATA194400.012.6215091
GTCCTAG84600.012.5277561
GTATTAA162900.012.5220671
TATACAG145500.012.3591565
TACACTG147000.012.2823695
GTATTAT179400.012.2201161
GTCGTAC5351.0942067E-412.2117961
TCTATAC107400.012.1540263
GTATAAG103550.012.12793351
Produced by FastQC (version 0.11.5)