FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19742626
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC17800.032.1744145
CCTATAC78600.015.3159533
TATACTG200800.014.4806285
GTATATA382250.013.7685151
GTATTAG142350.013.3610461
GTCCTAG95600.013.1366561
CTATACT140300.012.9217734
CGTACGA6804.7872345E-712.7961445
GCCGACC16650.012.626619145
GTATAGG74700.012.5361951
TCCGTAC29050.012.4819473
ACTGATC154600.012.475288
CCTACAC92300.012.4140613
CCGTACG8202.7481292E-812.380954
GGATCGT29350.012.3520788
TCTATAC134350.012.3070983
GCTAGAC107900.012.1650973
CTATACA160250.011.9466374
GTCCTAC61500.011.9218281
GTATAGA134800.011.9014251
Produced by FastQC (version 0.11.5)