FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26870761
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG129950.016.1427461
TATACTG215900.015.5834715
GTATAGA195800.014.9028441
GTATTAG201200.014.8997121
GTATATA360500.014.5978371
GTATAAG193500.012.8292411
CTATACT176750.012.7180984
TACACTG217800.012.6842855
CCTATAC123950.012.63707353
TATACAG245350.012.5899035
GTACTAG76650.012.3108221
GTGTATA256500.012.2816911
TCTATAC188450.012.2753483
GTATAAT266000.012.2250941
CTAGGAC100900.011.9304323
GTATTAA259150.011.90401651
GTATATG268800.011.827711
GTGTAGG147600.011.7535041
TATATAC370150.011.6175953
GTATTAC178100.011.4932011
Produced by FastQC (version 0.11.5)