FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19070880
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA5904.9094524E-915.9769275
CCTATAC73450.014.9086873
CGACGAA7907.094059E-1114.682353145
TATACTG177600.014.2898265
GTATATA330100.014.1617291
GTATTAG125850.014.0738371
GTATAGA123550.012.9845071
GTATAGG74050.012.6456321
GTATTAT199950.012.6337931
GTATAAG116850.012.6108171
GTATTAA177450.012.5585161
GTCCTAG94800.012.5577381
CCGTACG7108.0708924E-712.2561594
TCTATAC120350.011.9309043
CTATACT125500.011.7873974
GTATAAT194300.011.58151
TATACAG169500.011.550375
GTGTATA223050.011.520651
TACACTG170350.011.3224755
TATACCG13451.8189894E-1211.3213675
Produced by FastQC (version 0.11.5)