FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36659237
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG266800.015.4814471
GTATAGG157550.015.4812481
TATACTG350150.014.7237775
GTATATA642300.014.50019551
GTATAGA255050.014.0885081
GTATTAT402650.013.2328521
TATACAG345600.013.1552025
TCTATAC238150.012.9415393
GTATTAA359650.012.8975111
CTAATAC263150.012.897043
ACTGATC342200.012.7341798
CCTATAC149600.012.5064963
GTGTATA425550.012.486641
GTATAAG242850.012.1060371
GTATATG378300.012.0505921
TTATACT391950.012.0441454
ATAATAC407000.012.00917153
ATTATAC360850.011.9172363
TAATACT369400.011.837124
GTATAAT380750.011.7061361
Produced by FastQC (version 0.11.5)