FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16592409
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC18500.027.822418145
GCCGACC14100.022.108416145
GTATAGG62700.014.5882961
GTATATA304650.014.2733921
TATACTG160050.014.0446575
GTATTAG112050.013.4109651
GTATAAG108750.012.4828541
GTCCTAG80200.012.40074351
CTGCCGC57700.012.312848145
GTATAGA110000.012.2750081
TATACCG11251.0913936E-1112.2463375
GACGACA24350.012.206556145
CTATACT107900.012.164634
CCTACAC82900.012.159633
TTATACT179850.012.1366224
CCTATAC65900.011.9950263
CCTAGAC94950.011.9148823
GTCCTAA88450.011.8186041
GTATTAA162150.011.7744421
CTAATAC115800.011.71097853
Produced by FastQC (version 0.11.5)