FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14198252
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC13350.016.29081145
GTATAGG56200.014.72600651
GTATTAG102850.014.681671
TATACTG123350.014.4619095
GTATAGA93300.014.1614011
GTATATA218850.013.4014341
GTATAAG90250.012.9507551
TATACAG125850.012.9069755
TAGACTG96850.012.5788035
GTATAAT147350.012.31703951
GGATCGT24300.012.2340578
TAATACT138900.012.1127124
GTATTAT147450.012.0625111
CTATACT92800.012.034514
CCTATAC56650.012.0337773
GTGTATA153900.011.9815091
ATAATAC146450.011.884913
GTACAGG86200.011.8748541
GTACTAG47350.011.8055761
CTTACAC101100.011.6208513
Produced by FastQC (version 0.11.5)