FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20909378
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC12050.022.861423145
TATACTG167850.014.5153155
GTATATA311450.014.1005131
GTATTAT204700.014.0425271
GTATTAG153250.013.8782611
GTATAGG80850.013.6468211
TCTATAC131250.013.3711643
GGATCGT35000.013.0513388
GTATAAG134650.012.9381671
GTATAGA133800.012.8576071
GTCCTAT93350.012.5192821
GTATTAA197150.012.4447971
GTGTATA233250.012.354831
GATCGTT37500.012.1817159
GTATACG19200.012.0980851
CCTATAC80950.012.0940043
CTATACT136200.011.9795334
CTATACC90900.011.8068044
GTATTAC102800.011.7921041
TATATAC308100.011.7217063
Produced by FastQC (version 0.11.5)